Starch-iodine assay method underestimates α-amylase inhibitory potential of antioxidative compounds and extracts

Unlocking the potential of dietary starch with exogenous α-amylase

Potential Anti-obesity Effects of some Medicinal Herb: In vitro α-Amylase, α-Glucosidase and Lipase Inhibitory Activity

In this study, in vitro inhibitory activity of methanolic and chloroform extracts of some medicinal plants including C. wightii, T. ammi, N. sativa, C. arabica, L. usitatissimum, C. cyminum, and R. Graveolens was evaluated toward α-glucosidase, α-amylase, and lipase comparing with acarbose and orlistat as the standard inhibitors. Our results revealed that both methanolic and chloroform extracts...

Interferences with the starch-iodine assay for serum amylase activity, and effects of hyperlipemia.

Recent reports imply or claim that amylase activity is inhibited in hyperlipemic sera, because diluted samples showed greater activities when assayed by a starch-iodine method. We find that dilutions of both clear and lipemic samples give higher-than-expected activities when assayed by a starch-iodine method, an effect attributable to the variable effects of protein, turbidity, and triglyceride...

Inhibitory Effects of Swietenia Mahagoni Seeds Extract on Α-Glucosidase and Α-amylase

This study analyzed the inhibition activity of Swietenia mahagoni seeds extract on α-glucosidase and α-amylase enzymes inhibition assays.  Swietenia mahagoni seeds were extracted by using Supercritical Carbon Dioxide (SC-CO2) extraction at pressures of 20- 30 MPa and temperatures of 40- 60°C.  The oil yields obtained were analyzed with α- glucosidase and α- amylase enzymes inhibition assays.  A...

In vitro α-amylase and α-glucosidase Inhibitory and Cytotoxic Activities of Extracts from Cissus cornifolia Planch Parts

CONTEXT Hyperglycemia is the hallmark of type 2 diabetes mellitus, and its prevention will go a long way in managing the disease and its associated complications. Reduction of postprandial hyperglycemia through retarding carbohydrates digesting enzymes is one of the major therapeutic approaches used in the management of diabetes. OBJECTIVE The aim of the present study was to investigate the a...